Resumo

SULBARAN, María Zulay et al. Hepatitis C virus (HCV) infection in hemodialysed patients seronegative for anti-HCV antibodies. Rev. Soc. Ven. Microbiol. [online]. 2015, vol.35, n.1, pp.51-55. ISSN 1315-2556.

Hepatitis C virus infection is common in hemodialysed patients. Sera from 43 patients from the Dialysis Unit “Dr. José Maza Carvajal” of the University Hospital “Antonio Patricio de Alcalá”, in Cumana, Sucre state, were evaluated. Antibodies against HCV (anti-HCV) were determined using three immunosorbent assays. The 5 ‘non-coding (5’NC) HCV region was amplified by RT-PCR in all samples. The presence of antibodies and HCV RNA was 9.3% and of HCV RNA in seronegative sera 42%, which represents a frequency of infection of 51%. Phylogenetic analysis of the 5’NC region showed that genotype 2 was the most frequently found, particularly due to subtype 2b, followed by genotype 1, while seven subtypes could not be determined. The presence of a high number of seronegative HCV-infected hemodialysed patients might be due to the immunocompromised condition of these patients; hence the importance of determining the viremia. 

Palavras-chave : HCV; ELISA; RT-PCR; RNA; genotype; hemodialysis.

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