Abstract

GUEVARA, Armando; SAHAI, José Miguel  and  TEDESCO-MAIULLARI, Rosa. Clonal persistence of Pseudomonas aeruginosa producing metallo-β-lactamases in a hospital in Ciudad Bolivar, Venezuela. Rev. Soc. Ven. Microbiol. [online]. 2015, vol.35, n.2, pp.77-82. ISSN 1315-2556.

Pseudomonas aeruginosa is one of the species most frequently isolated in associated health care infections with an important role as pathogen. The clonal relation of P. aeruginosa producing metallo-β-lactamases (MBLs) isolated from patients hospitalized at the Hospital Universitario “Ruiz y Páez” Complex of Ciudad Bolivar, Venezuela, for the years 2008 to 2014, was determined. A set of 10 isolates of P. aeruginosa producing MBLs were evaluated. The antimicrobial susceptibility was performed by the disk diffusion test. MBLs production was determined by the method of carbapenem disks combined with ethylene-diamine-tetra-acetic acid-sodium mercapto-acetic. By means of the polymerase chain reaction test the presence of the genes encoding for MBLs of the families: IMP, VIM and SPM were investigated. Clonal spread by Random Amplified Polymorphic DNA (RAPD) was investigated. All strains were multiresistant and the presence of VIM type MBLs was demonstrated. By the RAPD the different strains were classified into three distinct clonal groups, highly related. In conclusion, all strains of P. aeruginosa were multiresistant, producing VIM type MBL and the clonal dissemination and persistence were demonstrated.

Keywords : Pseudomonas aeruginosa; metallo--lactamases; molecular epidemiology; PCR; RAPD.

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