Abstract

MORENO, Xiomara et al. Frequency and in vitro susceptibility profile of Candida parapsilosis Complex isolates from patients with candidemia. Rev. Soc. Ven. Microbiol. [online]. 2015, vol.35, n.2, pp.103-110. ISSN 1315-2556.

The aim of this study was to determine the frequency and in vitro susceptibility profile of Candida parapsilosis Complex isolates from patients with candidemia. Seven hundred and fifty four (754) strains (Period 2008-2011), from the Candidemia Surveillance Network of the Instituto Nacional de Higiene “Rafael Rangel” were studied. The strains identification was performed by phenotypic methods. In vitro antifungal susceptibility was evaluated by the Etest® method and minimum inhibitory concentration for amphotericin B (AB), caspofungin (CS), fluconazole (FZ), and voriconazole (VZ) was determined. Epidemiological cut off values (ECV) and ranges for wild type strains (WT) were also calculated for each antifungal. Forty three point six (43.6%) of the isolates (n=328) belonged to C. parapsilosis Complex; all of them were susceptible to AB and showed low resistance percentages to FZ (4.3%), VZ (1.2%) and CS (0.6%). The ECV and WT strains ranges (in mcg/mL) were: FZ: 2/0.03-2; VZ and AB: 0.06/0.002-0.06 and CS: 0.5/0.002-0.5 respectively. The results of this study provided important information about the behavior of the C. parapsilosis Complex against the most commonly antifungal agents used for the treatment of candidemias.

Keywords : Candida parapsilosis Complex; candidemia; antifungal agents; in vitro susceptibility testing; epidemiological cut off values; wild type strains.

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