Abstract

GUZMAN, Militza et al. Identification of genes encoding aminoglycoside-modifying enzymes of Klebsiella pneumoniae nosocomial isolates. Rev. Soc. Ven. Microbiol. [online]. 2016, vol.36, n.1, pp.10-15. ISSN 1315-2556.

Klebsiella pneumoniae infection is a growing problem in hospitals. The objective of this study was to evaluate resistance to aminoglycosides and detection of genes encoding for aminoglycoside modifying enzymes (AME) in hospital isolates of K. pneumoniae. Fifty-six isolates from patients with diagnosis of nosocomial infection at the University Hospital Antonio Patricio de Alcala, during the period January to September 2008 were included for study. Antimicrobial susceptibility was determined by the methods of diffusion and agar dilution, following the Institute for Clinical and Laboratory Standards Guidelines. Genes encoding AME were determined by the polymerase chain reaction procedure. Resistance results for Gentamycin were 33.9% and for Tobramycin 35.7%. Aminoglycoside resistance phenotypes most frequently identified were I (ANGMKTob) and II (GMKTob). The genes involved were aadA (21.4%), aac(3)-IIa (16.1%), aadB (14.3%), aac (6`)-Ib (3.6%) y aph (3`)-Ia (1.8%) For 10 of the isolates studied more than one gene was identified. In 13 isolates the phenotype corresponded to the genes found. Aminoglycoside resistance in the isolates studied is mainly due to the presence of acetyltransferase enzymes.

Keywords : Klebsiella pneumoniae; acetyltransferases; adenyltransferases; aminoglycoside; resistance.

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