Abstract

PONCE DE LEON, Patricia; PINTAGRO, Estefanía; LOPEZ MURUA, Gabriel  and  RACCA, Liliana. Desialylation study produced by muscle larvae of Trichinella spiralis. Rev. Soc. Ven. Microbiol. [online]. 2016, vol.36, n.1, pp.29-34. ISSN 1315-2556.

Trichinella spiralis is the cause of an endemic zoonosis in Argentina. The objective was to study the erythrocyte desialylation by T. spiralis muscle larvae (ML) applying an aggregation titulation method. We worked with ML concentrates, which were incubated with an equal volume of O Group erythrocytes (RBCs) at 37° C for 3 hours, (with and without controlled agitation) and for 150 minutes, taking samples at 30 minutes intervals to study the course in time of the desialylation. RBCs control were incubated with an equal volume of physiological saline solution. Aggregation titulation method was applied and the title and total score coefficient (TSexpC) were calculated. The results showed that erythrocytes incubated with ML showed more aggregation than controls. The average TSexpC with agitation (0.43) was significantly lower than when erythrocytes were not stirred (0.72). The course in time of the erythrocyte desialylation showed that the RBCs contol title decreased significantly at 90 minutes in 5/10 repetitions and at 150 minutes in 9/10. Increasing the incubation time produced an increase in erythrocyte desialylation, except at the 120 and 150 minutes interval where no significant differences in TSexpC values were found. The in vitro experience would suggest that in cases of in vivo infection, ML could capture sialic acid from sialylate residues present in the muscle cell.

Keywords : Trichinella spiralis; muscle larvae; desialylation; human erythrocytes; Polybrene method.

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