Resumo

ARAUJO, Karelen et al. Intergenic fusion of Saccharomyces cerevisiae and Hanseniaspora guillermondii protoplasts. Rev. Soc. Ven. Microbiol. [online]. 2016, vol.36, n.2, pp.51-57. ISSN 1315-2556.

Protoplast fusion has facilitated the development of new yeast strains with very interesting biotechnological properties. The main objective of this research was to obtain hybrid yeast with potentialities of two different genera, that could be used in the wine manufacture. Saccharomyces cerevisiae protoplasts from the Zulian region were fused with  Hanseniaspora guillermondii CECT 11102 of commercial origin. Saccharomyces is a yeast that produces high levels of ethanol but the aromatic profile is simple and common. Hanseniaspora does not withstand ethanol concentrations, but it can generate pleasant and intense aromas. Identification of yeast before and after the fusion of protoplasts was performed using the PCR-RFLP technique of the 5.8S rDNA gene and the adjacent ITS1 and ITS2 intergenic regions of the extracted DNA, subjecting the amplified products to a restriction with the enzymes HinfI, HaeIII, CfoI and DdeI. Polyethylene glycol was used to induce fusion of protoplasts. The hybrid strain showed characteristics of both parental yeasts because it resisted high concentrations of ethanol as S. cerevisiae and was able to metabolize the salicin as H. guillermondii. PCR-RFLP molecular analysis of hybrid yeast showed a different band pattern than those pertaining to parental yeasts.

Palavras-chave : protoplast fusion; Saccharomyces cerevisiae; Hanseniaspora guillermondii.

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