Abstract

RIOS VELASQUEZ, Maricruz del Valle; SAAVEDRA RODRIGUEZ, Meybe Carolina; DEL VENTURA VILLARROEL, Fabiola  and  SALAS MORA, Rosa Alba. Detection of Guanarito virus by one step RT-PCR. Rev. Soc. Ven. Microbiol. [online]. 2017, vol.37, n.2, pp.82-86. ISSN 1315-2556.

A one-step RT-PCR was standardized for the detection of Guanarito virus (VGTO), with the purpose of replacing the cell culture technique, used for the diagnosis of Venezuelan hemorrhagic fever, and to minimize the biological risk from exposure to this virus. Specific primers were designed for the detection of the S segment of the nucleocapsid gene of VGTO. A protocol was standardized where 44 samples were analyzed: 18 acute sera, 11 macerated tissues and 15 culture supernatants in Vero-E6 cells as positive controls. The results obtained by the RT-PCR were compared with the cell culture technique, finding 100% sensitivity and specificity, with positive and negative predictive values of 97.7% and 100%, respectively. The Pearson correlation coefficient between both tests was 99%. This is the first report of the use of a methodology for the molecular diagnosis specific to VGTO in Venezuela.

Keywords : Guanarito virus; RT-PCR; Venezuelan hemorrhagic fever.

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