Abstract

JIMENEZ, Odaliz  and  CONTRERAS, Nancy. Detection Xanthomonas albilineans, causal agent of sugarcane leaf scald, using Elisa technique and selective media. Bioagro [online]. 2008, vol.20, n.3, pp.209-213. ISSN 1316-3361.

Leaf scald sugarcane, caused by Xanthomonas albilineans (Ashby) Dowson, can present different types of symptoms due to its systemic character. Because in the phase of latency the infected plants may lack external symptoms, which makes difficult the diagnosis of the disease and favors its dissemination, the objective of this research was to detect X. albilineans in symptomatic and asymptomatic samples by means of the technique of indirect ELISA and two selective means (XAS y modified of Wilbrinks). The technique of indirect ELISA allowed 98.22 % of detection, while the use of  XAS and modified means of Wilbrinks allowed a frequency of detection of 97.33 and 61.78 %, respectively. In asymptomatic samples the detection was 18.75 % by means of the ELISA technique, and 15.18 and 8.93 %, respectively, for XAS and modified of Wilbrinks. Technique of indirect ELISA and selective means XAS could be used as a diagnostic method of for X. albilineans detection in symptomatic and asymptomatic samples of sugarcane.

Keywords : Bacteria; diagnostic method; Saccharum officinarum.