Resumo

BITTARA, Francisco et al. Molecular and phytochemical characterization of eight potato (Solanum tuberosum L.) genotypes and its relationship with infection by Spongospora subterranea (Wallr.) Lagerh. Bioagro [online]. 2013, vol.25, n.1, pp.11-22. ISSN 1316-3361.

Potato powdery scab is a disease caused by Spongospora subterranea, whose control should be realized by integrating several practices, of which, plant resistance is probably the main component. The present study was conducted to determine the molecular and phytochemical characteristics of eight potato genotypes and the relationship with their reactions to infection by S. subterranea. Reactions to infection were evaluated with vitro plants of the cvs. Granola, Esperanza, Kennebec and the clones 393180-32, 393194-1, 393193-16, 392634-21 y 392636-31, planted in plastic bags and inoculated with sporesores obtained from a potato field. Evaluation was realized by quantifying root zoosporangia and phytochemical analyses after 8 weeks. Molecular characterization, by using repeated simple sequences (RSS), was performed in six-week-old vitro plants. Twenty RSS nuclear flank primers were used. Results showed differences among potato genotypes with regard to susceptibility to the pathogen, where the clones 392636-31 y 393193-16 had the lowest amount of zoosporangia per squared millimeter of root. Primer STM-1016 allowed a classification of the clones very similar to the one obtained by analysis of infection, locating clones 393193-16 in the same 392636-31 group. Phytochemical analysis showed differences among genotypes respect to secondary metabolites, suggesting an association between flavonoids and the degree of infection of the materials.

Palavras-chave : Zoosporangia; repeated simple sequence; flavonoids; susceptibility.

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