Resumo

MUNOZ-BAENA, Laura; GUTIERREZ-SANCHEZ, Pablo A  e  MARIN-MONTOYA, Mauricio. Detection and genome sequencing of Potato virus Y (PVY) infecting tomato in Antioquia, Colombia. Bioagro [online]. 2016, vol.28, n.2, pp.69-80. ISSN 1316-3361.

PVY is one of the most limiting viruses in solanaceous plants. These crops are frequently planted together facilitating their cross-inoculation with this virus. Recent studies in Antioquia, Colombia, detected high levels of incidence and genetic variability of PVY infecting potato and tamarillo. In this study, Next-Generation Sequencing (NGS) transcriptome analysis of tomato leaves was used to confirm the presence of PVY in tomato crops in that region; virus incidence was evaluated in three plots using TAS-ELISA and further confirmed by real-time RT-PCR (RT-qPCR). TAS-ELISA of 45 leaf samples detected PVY in 13.3 % of them; RT-qPCR confirmed  the  serological  results  with  threshold  cycle  values in the 14.31 and 33.8 range and a Tm of 77.5 ± 1.5 °C.  Bioinformatic analysis confirmed  the  presence  of two PVY variants (proportion 3:2),  sharing 97.5 % of sequence identity. Both genomes are 9726 nt in size and code for 3061 amino acids polyprotein flanked by 5´and 3´ untranslated regions (UTR) of 218 and 325 nt. Phylogenetic analysis of both the sequences coding for the coat protein and the complete polyprotein suggest phylogenetic affinity with PVY^NTN. To our knowledge, this is the first American report of a complete PVY genome naturally infecting tomato.

Palavras-chave : Deep sequencing; NGS; Potyvirus; Real-time RT-PCR; Solanum lycopersicum; TAS-ELISA.

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