Abstract

ARIAS, Johanna M et al. Characterization of a wild strain of Saccharomyces cerevisiae for obtaining a biopreparation with probiotic profile. Bioagro [online]. 2017, vol.29, n.3, pp.197-206. ISSN 1316-3361.

Generation of organic wastes is a worldwide environmental issue, although, if properly handled they may be a valuable source of animal nutrients. The objective was to determine the performance of a wild strain Saccharomyces cerevisiae in a biopreparation from ruminal content enriched with peels of fruits. Probiotic properties of the wild strain such as tolerance to bile salts, pH changes, changes in temperature, high concentrations of sodium chloride; additional to antagonism testing and gas production from glucose were verified in vitro. Microbial growth was evaluated in a medium prepared from clarified ruminal content 40 % v/v, enriched with peel wastes from papaya, pineapple and banana at 25, 50, and 75 % w/w concentrations. Results showed that wild strain had a higher growth in the medium obtained with 50 % w/v fruit wastes. Growth of the strain in this medium disclosed the highest biomass production at 40 hours (2.28x10⁸ cfu·mL^-1). The viability of the strain showed no important decrease during two months in the log scale of the inoculum. It is concluded that ruminal content enriched with fruit peels provides necessary nutrients for growth of the wild strain S. cerevisiae with probiotic characteristics and the biopreparation represents a supplement which may be useful not only at nutritional level but also to decrease environmental impact caused by these organic wastes.

Keywords : Ananas comosus; Carica papaya; Musa sapientum; ruminal content.

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