Resumo

BARRIOS, Emilia E et al. Effect of Treatment with Praziquantel on the activity of alkaline phosphatase acid phosphatase, superoxide dismutase in Crude Extracts and Excretion-secretion Products of Schistosoma mansoni worms.. Salus [online]. 2013, vol.17, suppl.1, pp.46-55. ISSN 1316-7138.

Venezuela is among South American countries affected by schistosomiasis and chemotherapy with praziquantel (PZQ) is the main control strategy. We determined the quantitative effect of treatment with PZQ on alkaline phosphatase activity (ALP), acid phosphatase (ACP) and superoxide dismutase (SOD) in soluble antigens of worms (SWAP) and excretion-secretion products (EEP) of male and female worms (SMWAP_c, SFWAP_c, ESPWM_c and ESPWH_c) or incubated with PZQ in vitro (SMWAP_PZQ, SFWAP_PZQ, ESPWM_PZQ and ESPWH_PZQ). Total proteins were determined by colorimetry, SOD and ACP by spectrophotometry and fluorometry ALP. There was higher protein concentration in the untreated worms EG, and the preparations obtained after incubation with PZQ in vitro, in the EG, an increase in ACP activity in the EG and PG prepared with non-treated worms and a decrease of such activity on the EG and treated PG. On the other hand, SOD activity, the EG showed statistical significance in the treated worms. In the PG showed the same behavior, but those differences were not statistically significant. Similarly, there was a decrease in the concentration of ALP noticeable in the EG and worm PGh treated worms relative to untreated statistically significant. In conclusion, we observed a decrease in total protein, ACP and SOD enzyme activities and concentration of ALP, and EG in PG treated worms.

Palavras-chave : Praziquantel; enzymes; Schistosoma mansoni; crude extracts; excretory-secretory products.

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