Resumen

GARCIA, Marlén; GONZALEZ, Isabel; HENRIQUEZ, María  y  JHON D, Jesús. Assessing amoebiasis diagnosis in notifying laboratories of Carabobo State. Salus [online]. 2015, vol.19, n.1, pp.20-26. ISSN 1316-7138.

For decades the diagnosis of amoebiasis has been based on microscopic analysis. However, due to the existence of nonpathogenic amoebae morphologically similar to E. histolytica, for a more accurate diagnosis WHO provides some criteria for this procedure. Ignorance about analysis criteria has led to an overestimation of this parasitic infection. Through a descriptive, nonexperimental and cross-sectional survey and prior validation and informed consent, analysts participating in an interview represented the sample which consisted of 20 laboratories distributed in 10 municipalities of Carabobo State. Absolute and relative frequencies obtained by statistical data processing software (SPSS 11.0) showed that 65% of laboratories have appropriate infrastructure and reference material. 15% have equipment for serological diagnosis. None of the laboratories has a microscope with a micrometer. 65% of the staff has to rotate through two work areas, and 85% processes stool samples after two hours of reception. 45% of analysts apply sample rejection criteria and only 40% provide instructions about proper collection. 100% omitted the use of concentration methods, special stains and using immersion objective. Only 10% reported E. histolytica/dispar complex and 20% mentioned reporting E. hartmanni. 75% of analysts are unaware of WHO guidelines. We conclude that in the assessed laboratories, compliance with regulations for a proper and accurate diagnosis of E. histolytica is poor.

Palabras clave : Entamoeba histolytica; Entamoeba dispar; Entamoeba hartmanni; trophozoites; chromatoid bodies.

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