Resumen

LUIGI, Teresita; ROJAS, Legna  y  VALBUENA, Oscar. Polymerase Chain Reaction (PCR) for identification of Salmonella spp. using the invA gene. Salus [online]. 2015, vol.19, n.3, pp.41-46. ISSN 1316-7138.

A PCR protocol was developed to identify strains of Salmonella from different sources using a set of “primers” PCR specific to Salmonella, for the recognition of 119 bp invA gene sequence. The results met the maxims of selectivity: inclusiveness, all strains of Salmonella presented positive signal for the gene, exclusivity since no strain Salmonella (Shigella, E. coli, E. coli O157: H7) showed no signal positive robustness because in the 14 strains of Salmonella expected signal was obtained in a total of four repetitions with equal working conditions in four non-consecutive days. Also it was reproducible, results were identical by modifying the reaction conditions. Comparing PCR with conventional culture, using non-selective pre-enrichment in peptone water and selective enrichment in selenite-cystine broth, it was found that the PCR was 100% sensitive and specific, with 100% of inclusiveness, 100% exclusive and 100% of both positive and negative predictive value.

Palabras clave : Salmonella spp ; Polymerase Chain Reaction (PCR); gene invA.

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