Abstract

RIVERO, José et al. DNA degradation of Anopheles darlingi collected at high relative humidity and preserved in isopropanolDegradación de ADN de Anopheles darlingi colectados a una humedad relativa alta y preservados en isopropanol. Bol Mal Salud Amb [online]. 2007, vol.47, n.1, pp.149-151. ISSN 1690-4648.

Anopheles darlingi mosquitoes, exposed to variable relative humidity (RH) in the field, were preserved during transportation to the laboratory using two different methods: 100% isopropanol at ambient temperature or frozen in liquid nitrogen or dry ice. The DNA isolated from samples collected at RH greater than 91% and preserved in isopropanol was degraded, while DNA isolated from insects kept in liquid nitrogen or dry ice maintained its integrity when collected under conditions of up to 95% RH

Keywords : Anopheles darlingi; relative humidity; mosquito preservation; DNA integrity; mosquito transportation.