Abstract

MARTIRADONNA OCHIPINTI, Gianna; SOTO VIVAS, Ana  and  GONZALEZ, Julio. Breeding protocol for Musca domestica in the laboratory. Bol Mal Salud Amb [online]. 2009, vol.49, n.2, pp.317-319. ISSN 1690-4648.

We present a method of growing Musca domestica under laboratory conditions. At a temperature of 32 ºC, relative humidity of 70 to 80% and photo period of 12 hours of light, the life cycle of M. domestica lasts about 9 days. The diet for the adult fly consists of 80 grams of commercial sugar, 250 mL of pasteurized milk and filtered water. The culture medium for larvae was made with strips of paper napkins moistened with a solution of milk and yeast that were placed in layers to 5 cm deep in a glass bottle filled 1/3 full. About 250 larvae eggs were placed on the top layer and then a last layer of dry strips of paper was added. After five days the pupae were removed from the rearing flask. These were placed into a clean cage and four days later the adults emerged.

Keywords : Musca domestica; biological cycle; larvae; culture medium.