Resumo

BONA, Ana Caroline Dalla; TWERDOCHLIB, Adriana Lacerda  e  NAVARRO-SILVA, Mário Antônio. Detection of dengue virus in natural populations of mosquitoes. Bol Mal Salud Amb [online]. 2011, vol.51, n.2, pp.107-116. ISSN 1690-4648.

Dengue is caused by Flavivirus which exhibits high genetic diversity, with four serotypes and various genotypes. The disease is endemic in most countries in the Americas, and the female Aedes (Stegomyia) aegypti (Linnaeus, 1762) are the only transmitters of epidemiological importance. A single infected mosquito remains so for the rest of his life and can infect multiple human hosts. For dengue virus detection in mosquitoes, the circulating serotype can be revealed or detect an entry of a new serotype in the region, without any ethical implications, and reproducible results. This review covers the techniques for detecting virus in mosquitoes, their advantages and limitations, as well as previous studies with natural populations of Aedes aegypti.

Palavras-chave : RT-PCR; real time PCR; Virus isolation; Flavivirus.