Abstract

MOLINA DE FERNANDEZ, Darjaniva; BASTIDAS B, Danny M  and  FIGUEROA ACOSTA, Luisa Elena. Malathion vs.  Aedes aegypti  (Linnaeus, Diptera: Culicidae) from different regions of Venezuela. Bol Mal Salud Amb [online]. 2013, vol.53, n.1, pp.46-55. ISSN 1690-4648.

In  Venezuela,  malathion  has  been  widely  used continuously  in  control  programs  for  Aedes  aegypti. Therefore,  a  study  in  mosquitoes  from  urban  areas with  high  dengue  casuistry  in  the  states  of  Amazonas, Aragua, Bolivar, Lara, Merida and Zulia was conducted to  determine  the  status  of  this  vector  susceptibility  to malathion,  compared  with  the  reference  susceptible strain,  Rockefeller.  Bioassays  were  done  on  bottles treated  with  the  insecticide  malathion,  100ug/mL evaluating  the  diagnostic  doses  in  30  minutes  and biochemical  assays  in  microplates  were  performed  to determine metabolic mechanisms associated with status against  insecticide.  The  bioassay  results  showed  that there  is  malathion  susceptibility,  which  was  confirmed by  biochemical  tests.  However,  significant  differences were  found  among  all  strains  assessed  values    of P<0.005  for  esterases  alpha  (α),  beta  esterases  (β) and standard acetylcholinesterase (AChe) and inhibited acetylcholinesterase  (Achei).  The  mean  comparison test of Bonferroni showed similarity between the strains Rock, Amazon and Lara for esterases α and β. Similarity was  found  between  the  strains  Rock,  Bolivar  and  Zulia for the Ache and Achei tests. This study concludes that malathion  showed  its  potential  use  in  controlling  the dengue vector in the locations evaluated.

Keywords : Aedes  aegypti; esterases; malathion; susceptibility.

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