Resumen

GARRIDO, Oswgladys; GARATE, Teresa  y  FERRER, Elizabeth. Sequences analysis of molecules of Taenia solium post-transcriptionally processed by trans-splicing. Bol Mal Salud Amb [online]. 2015, vol.55, n.1, pp.41-51. ISSN 1690-4648.

Neurocysticercosis is a neurological disease caused by the presence of Taenia solium cysticerci in the central nervous system. T. solium uses an alternative mechanism for processing some mRNAs, known as trans-splicing, in which a small RNA molecule (Spliced Leader, SL) is added to the 5' end, of one pre-mRNA molecule, leading to the formation of different mature mRNAs that all contain a common 5' end. The aim of this study was to analyze the sequences of some of the molecules that undergo this type of post-transcriptional processing in order to learn more about this mechanism in T. solium. Expression libraries of T. solium cysticerci were screened using PCR with SL as the forward primer and ZAP 3' UP, an oligonucleotide that hybridizes to the vector sequence, as the reverse primer. Different cDNAs were obtained which were cloned in the pGEM-T-easy plasmid, sequenced and then compared with sequences in databases (GenBank). A total of 14 different molecules showing similarities to T. solium, Echinococcus sp. and Hymenolepis sp. proteins were obtained. Complete transcripts encoding a variety of proteins that are part of the biology of living organisms, such as enzymes, transporters and structural proteins, were also identified. Although we could not determine whether specific cDNA groups (with common functions) are selected to carry out this post-transcriptional modification, we were able to observe that the process of trans-splicing occurs in a variety of RNAs that code for several proteins biologically important for T. solium.

Palabras clave : solium; sequencing; Spliced Leader; trans splicing.

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