Resumen

SUNTASIG-NEGRETE, Edison Roberto; MORENO-LOPEZ, Joana Alexandra; CARRERA-SANCHEZ, Karina María Elena  y  MANOBANDA-PINTO, Paul Marcelo. Isolation of hydrocarbonoclastic bacteria in soils contaminated with lubricating oil in Puyo, Ecuador. Agroecología Global [online]. 2025, vol.7, n.13, pp.59-80.  Epub 31-Oct-2025. ISSN 2665-0290.  https://doi.org/10.35381/a.g.v7i13.4644.

The study sought to identify bacteria capable of degrading oils and fats in contaminated soil from two lubricators in Puyo, Pastaza. Native bacteria were isolated from soil samples from two lubricators, with four replicates performed for each, and characterized morphologically using Gram staining. The catalase tests were positive for the samples from lubricator 1, and hydrogen sulfide production was detected in the fermentation test. In contrast, the samples from lubricator 2 were negative in the catalase test. In addition, the strain identified as M2R1 corresponding to the sampling point of lubricator 2 demonstrated the ability to ferment glucose, lactose, and/or sucrose. The isolated bacteria could belong to the genera Bacillus, Pseudomonas, and Nocardia. Molecular tests are recommended to accurately identify the genus and species of the isolates for their potential use in the remediation of contaminated soils.

Palabras clave : Fermentation; morphotype; bacterial strain; contaminated soils; contaminating hydrocarbons; biodegradation.

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